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p6 buffer exchange columns  (Bio-Rad)


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    Bio-Rad p6 buffer exchange columns
    P6 Buffer Exchange Columns, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 638 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p6+buffer+exchange+columns/Micro+Bio-Spin+P-6+Gel+Columns/us12566116-849-8-13
    Average 96 stars, based on 638 article reviews
    p6 buffer exchange columns - by Bioz Stars, 2026-09
    96/100 stars

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    Buffer Exchange:

    Article Title: Method and apparatus of preparing a sample of one or more molecule(s) for imaging with a cryo-electron microscope
    Article Snippet: .. All proteins were desalted by passing through two P6 buffer exchange columns (7326222, Biorad), equilibrated with 200 mM ammonium acetate (pH 6.9). ..

    Article Title: Characterization of Gas-Phase Native(-Like) Proteins Using Structures for Lossless Ion Manipulations
    Article Snippet: .. All proteins were desalted by passing through P6 buffer exchange columns (7326221, Bio-Rad), equilibrated with 200 mM ammonium acetate. ..

    Article Title: Cryo-EM of soft-landed β-galactosidase: Gas-phase and native structures are remarkably similar
    Article Snippet: .. For native ESIBD, it was desalted by passing through two P6 buffer exchange columns (7326222, Bio-Rad), equilibrated with 200 mM ammonium acetate (pH 6.9) and diluted in 200 mM ammonium acetate (pH 6.9) to reach the concentration of 10 μM, and used without further purification. .. For the preparation of plunge-frozen cryo-EM samples, it was desalted, purified, and transferred into a buffer (25 mM tris, 50 mM NaCl, 10 mM EDTA, and 2 mM MgCl 2 ) using a Superdex 200 Increase 10/300 column (28990944, Cytiva Life Sciences).

    Article Title: [2503.22364] High-resolution cryoEM structure determination of soluble proteins after soft-landing ESIBD
    Article Snippet: GDH (G7882) was purchased from Sigma-Aldrich; the lyophilized powder was reconstituted in 200 mM ammonium acetate (pH 6.9) to a final concentration of 20 μM. β -Galactosidase,14 GroEL11, and RuBisCo38 were prepared as previously described. .. All proteins were desalted by eluting through two P6 buffer exchange columns (7326222, Bio-Rad), equilibrated with 200 mM ammonium acetate (pH 6.9). .. GDH (G7882) was purchased from Sigma-Aldrich; the lyophilized powder was reconstituted in 200 mM ammonium acetate (pH 6.9) to a final concentration of 20 μM.

    Article Title: Characterization of Gas-Phase Native(-like) Proteins Using Structures for Lossless Ion Manipulations.
    Article Snippet: High-resolution mobility-based ion separations in Structures for Lossless Ion Manipulations (SLIM) have been useful for ion mobility separations for a variety of molecular classes in the gas phase.. Here, we present multipass SLIM separations for gas-phase proteins in their near-native state exhibiting charge-state-dependent arrival time distributions using carbonic anhydrase (29 kDa), alcohol dehydrogenase (148 kDa), and apo-transferrin (79 kDa).. The experimental CCS values were obtained from calibration curves for the arrival times of Agilent Tune Mix ions.

    Article Title: Cryo-EM samples of gas-phase purified protein assemblies using native electrospray ion-beam deposition.
    Article Snippet: .. GroEL was reconstituted from lyophilized powder as described previously.33 All proteins were desalted by passing through two P6 buffer exchange columns (7326222, Biorad) and equilibrated with 200 mM ammonium acetate (pH 6.9). ..

    Article Title: Cryo-EM of soft-landed β -galactosidase: Gas-phase and native structures are remarkably similar
    Article Snippet: β -galactosidase (G3153-5MG) was purchased from Sigma Aldrich. .. The lyophilized powder was resuspended in 200 mM ammonium acetate (pH 6.9) to a final concentration of 50 μ M. For native ESIBD, it was desalted by passing through two P6 buffer exchange columns (7326222, Biorad), equilibrated with 200 mM ammonium acetate (pH 6.9) and diluted in 200 mM ammonium acetate (pH 6.9) to reach the concentration of 10 μ M and used without further purification. .. For the preparation of plunge-frozen cryo-EM samples, it was desalted, purified, and transferred into a buffer (25 mM Tris, 50 mM NaCl, 10 mM EDTA, 2 mM MgCl 2 ) using a Superdex 200 Increase 10/300 column (28990944, Cytiva life sciences).

    Article Title: Mass-selective and ice-free electron cryomicroscopy protein sample preparation via native electrospray ion-beam deposition
    Article Snippet: .. All proteins were desalted by passing through two P6 buffer exchange columns (7326222, Bio-Rad), equilibrated with 200 mM ammonium acetate (pH 6.9). ..

    Concentration Assay:

    Article Title: Cryo-EM of soft-landed β-galactosidase: Gas-phase and native structures are remarkably similar
    Article Snippet: .. For native ESIBD, it was desalted by passing through two P6 buffer exchange columns (7326222, Bio-Rad), equilibrated with 200 mM ammonium acetate (pH 6.9) and diluted in 200 mM ammonium acetate (pH 6.9) to reach the concentration of 10 μM, and used without further purification. .. For the preparation of plunge-frozen cryo-EM samples, it was desalted, purified, and transferred into a buffer (25 mM tris, 50 mM NaCl, 10 mM EDTA, and 2 mM MgCl 2 ) using a Superdex 200 Increase 10/300 column (28990944, Cytiva Life Sciences).

    Article Title: Cryo-EM of soft-landed β -galactosidase: Gas-phase and native structures are remarkably similar
    Article Snippet: β -galactosidase (G3153-5MG) was purchased from Sigma Aldrich. .. The lyophilized powder was resuspended in 200 mM ammonium acetate (pH 6.9) to a final concentration of 50 μ M. For native ESIBD, it was desalted by passing through two P6 buffer exchange columns (7326222, Biorad), equilibrated with 200 mM ammonium acetate (pH 6.9) and diluted in 200 mM ammonium acetate (pH 6.9) to reach the concentration of 10 μ M and used without further purification. .. For the preparation of plunge-frozen cryo-EM samples, it was desalted, purified, and transferred into a buffer (25 mM Tris, 50 mM NaCl, 10 mM EDTA, 2 mM MgCl 2 ) using a Superdex 200 Increase 10/300 column (28990944, Cytiva life sciences).

    Purification:

    Article Title: Cryo-EM of soft-landed β-galactosidase: Gas-phase and native structures are remarkably similar
    Article Snippet: .. For native ESIBD, it was desalted by passing through two P6 buffer exchange columns (7326222, Bio-Rad), equilibrated with 200 mM ammonium acetate (pH 6.9) and diluted in 200 mM ammonium acetate (pH 6.9) to reach the concentration of 10 μM, and used without further purification. .. For the preparation of plunge-frozen cryo-EM samples, it was desalted, purified, and transferred into a buffer (25 mM tris, 50 mM NaCl, 10 mM EDTA, and 2 mM MgCl 2 ) using a Superdex 200 Increase 10/300 column (28990944, Cytiva Life Sciences).

    Article Title: Cryo-EM of soft-landed β -galactosidase: Gas-phase and native structures are remarkably similar
    Article Snippet: β -galactosidase (G3153-5MG) was purchased from Sigma Aldrich. .. The lyophilized powder was resuspended in 200 mM ammonium acetate (pH 6.9) to a final concentration of 50 μ M. For native ESIBD, it was desalted by passing through two P6 buffer exchange columns (7326222, Biorad), equilibrated with 200 mM ammonium acetate (pH 6.9) and diluted in 200 mM ammonium acetate (pH 6.9) to reach the concentration of 10 μ M and used without further purification. .. For the preparation of plunge-frozen cryo-EM samples, it was desalted, purified, and transferred into a buffer (25 mM Tris, 50 mM NaCl, 10 mM EDTA, 2 mM MgCl 2 ) using a Superdex 200 Increase 10/300 column (28990944, Cytiva life sciences).



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